The auction of frequencies in the 700 MHz and 3.4-3.5 GHz bands is aimed at boosting competition in a market where high prices have long been a gripe of customers and politicians.
Interested bidders have until Sept. 30 to apply, CTU said.
The regulator said that after public consultations it had reserved a section of 2x10 MHz in the 700 MHz band for new entrants.
CTU also set spectral limits in both bands and said existing operators would need to provide national roaming services for newcomers should they succeed in the auction.
O2 Czech Republic, Deutsche Telekom's T-Mobile and Vodafone<\/a> are the country's existing operators.
The planned auction for new 5G frequencies has been hit by delays as the state seeks to attract a fourth operator.
In January, the former head of CTU resigned over government changes to the auction, saying they risked slowing the rollout of the technology and were likely to result in court disputes.
Last month, O2 Czech Republic complained to the European Commission<\/a> over some 5G auction<\/a> conditions.
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